(2016). Cinc. doi: 10.1007/s00253-003-1289-4, Olsen, J. L. (2016). An example is the potential presented by the introduction of the bacterium Wolbachia as an endosymbiont of the mosquito Aedes aegypti, which transmits diseases such as dengue, yellow fever, chikungunya, and the more recently detected Zika virus (Walker et al., 2011). BioControl 58, 715722. LWT Food Sci. Past and future of non-Saccharomyces yeasts: from spoilage microorganisms to biotechnological tools for improving wine aroma complexity. J. The potential of fungal chitosan, when present in bioactive filters, to chelate heavy metals and inhibit pathogenic microbial agents in contaminated water was recently evaluated (e.g., Ruholahi et al., 2016; Tayel et al., 2016a) for the development of a renewable, ecofriendly, and cost-effective polymer that can help overcome the current problems of chemical and microbial water pollution (Tayel et al., 2016b). Technol. These toxins, previously mentioned in this text, are inactive crystals that, when ingested by Aedes larvae, are solubilized by intestinal proteases and converted into active toxins that interact with the cell membrane of the midgut, leading to pore formation, cell lysis, septicemia, and finally death (Gill et al., 1992; de Maagd et al., 2001). B. burgdorferi is not easily cultured in the laboratory, and the initial symptoms can be very mild and resemble those of many other diseases. Authors Periasamy Anbu 1 , Subash C B Gopinath 2 , Bidur Prasad Chaulagain 3 , Thean-Hock Tang 4 , Marimuthu Citartan 4 Affiliations Sci. Drug Discov. doi: 10.1146/annurev.micro.62.081307.162918, Ma, Y.-M., Liang, X.-A., Zhang, H.-C., and Liu, R. (2016). One example is the synthesis by E. coli of acetoin, which is responsible in part for a buttery aroma, using glucose as a substrate (Nielsen et al., 2010). doi: 10.1002/(SICI)1097-0290(19960705)51:1<126::AID-BIT15>3.0.CO;2-O, Tayel, A. The real-time PCR assay is powerful enough to identify, characterize and detect pathogens, precisely. Microbial characterization during composting of municipal solid waste. Biotechnol. Rev. Bior. The versatility of these microbes has enhanced the study and application of them in biotechnology, industry, and medical field. doi: 10.1016/S0734-9750(01)00086-6, Sheldon, R. A. (2016). Peroxidases, polyphenol oxidases, and tyrosinases obtained from microorganisms such as P. syringae, Arthromyces ramosus, and Agaricus bisporus may be applied to the removal of phenols, biphenols, and chlorophenols (Tatsumi et al., 1996; Tong et al., 1998; Akay et al., 2002; Kampmann et al., 2014). Sci. doi: 10.1016/j.soilbio.2008.03.011, Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., et al. Samples were found in different archeological sites dating to 20001200 BC (Samuel, 1996). Science 219, 632637. doi: 10.1016/S1369-5274(03)00040-7, Lugtenberg, B., and Kamilova, F. (2009). Actuatators B Chem. New York, NY: John Wiley & Sons, Inc. doi: 10.1002/0471238961.herbduke.a01.pub2, Durn, N., and Esposito, E. (2000). 1, 1015. Genetics :- Science of heredity study of what genes are, how they carry info, how they are replicated, and how expression of the info determines characteristics of the organism. Acad. 5, 399409. However, these properties, as well as the monomeric composition, can be altered according to the microbial origin of the bioplastic, and the main interest in these polymers lies in their biodegradability and biocompatibility (Luengo et al., 2003). Improvement of the mechanical properties of chitosan-alginate wound dressings containing silver through the addition of a biocompatible silicone rubber. Microbiol. Classic methods of studying gene function . The first indication of wine production comes from the presence of tartaric acid in an old jar, also dated to 54005000 BC, at the Neolithic site of Tepe in Mesopotamia (McGovern et al., 1996) and from grape juice residues, found in Dikili Tash in Greece and dated to 5000 BC (Valamoti et al., 2007). Trends Microbiol. Plant Cell Environ. Nature 517, 455459. Emir. *Correspondence: Luciana C. Vitorino, luciana.vitorino@ifgoiano.edu.br, Chemical and Fuel Technological Microbiology, http://www.who.int/immunization/sage/meetings/2014/april, Creative Commons Attribution License (CC BY), Laboratory of Agricultural Microbiology, Goiano Federal Institute, Gois, Brazil. They are poorly reactogenic, which is an advantage in terms of adverse effects but a disadvantage in terms of stimulating potent and long-lasting immune responses (Bobbala and Hook, 2016). The industry is now able to produce some microbial pigments for applications in food, cosmetics or textiles. doi: 10.1016/j.wasman.2015.12.032, Philip, S., Keshavarz, T., and Roy, I. doi: 10.4155/fmc-2016-0091. Small scale production and characterization of xanthan gum synthesized by local isolates of Xanthomonas campestris. In addition, the BAC biofilm can also biodegrade the cyanotoxins and organic substances that can change the taste and odor of potable water (Brown and Lauderdale, 2006). (2008). This promotion of growth by endophytic action may be a consequence of nitrogen fixation, synthesis of phytohormones, biocontrol of phytopathogens through the synthesis of antibiotics or siderophores, competition for nutrients, and the induction of systemic disease resistance (Ahmad et al., 2016). Thumbnail: A group of Genetically modified GloFish fluorescent fish. (2006). Acta 568, 200210. 37, 815. (2016). Live vaccine against measles, mumps, and rubella and the risk of hospital admissions for nontargeted infections. J. Bacteriol. (2016). Natl Acad. Natl Acad. Sci. Polym. Lipase from Rhizomucor miehei as a biocatalyst in fats and oils modification. The microorganisms are then grown on an industrial scale to synthesize products such as insulin, vaccines, and biodegradable polymers. 388, 287295. 64, 37893793. Microb. Microbial genetics provides powerful tools for deciphering the regulation, as well as the functional and pathway organization, of cellular processes. doi: 10.1016/j.crvi.2010.12.016, Simon, D., and Chopin, A. Cellulolytic and xylanolytic activities of common indoor fungi. Here we have also summarized the important applications of protease in different industries such as, paper mill, starch degrading sector, food processing factories, and detergent making companies. Res. Several transgenic species expressing Bt protein crystals, such as tomato, tobacco, and corn, have been cultivated worldwide because they have been successful in preventing the spread of caterpillars, especially Lepidoptera (Khan et al., 2016). 2, 396409. What are the biomarkers uncovered in proteomics used for? In the future, the microbiological processes for obtaining a variety of organic acids are expected to will be competitive, become established in the market, and allow for an annual increase in the production of these compounds. Raxibacumab for the treatment of inhalational Anthrax. 14, 137. doi: 10.1038/381480a0, McGovern, P. E., Zhang, J., Tang, J., Zhang, Z., Hall, G. R., Moreau, R. A., et al. doi: 10.1016/j.chom.2017.02.007, Wang, Z., Zhuge, J., Fang, H., and Prior, B. Ther. Nutr. This has been crucial in determining the structure-function relation of proteins, as well as for developing a better understanding of immune system reactions, cell biology, and signaling events. Recombinant (gene) vaccines are prepared from viruses engineered to carry genes encoding antigens from other disease-causing viruses for expression in the host after inoculation. doi: 10.1016/j.jip.2011.11.004, Paris, M., Tetreau, G., Laurent, F., Lelu, M., Despres, L., and David, J.-P. (2011). Vaccines, 5th Edn. Ind. In addition to bioethanol, other energy molecules such as biogas can be obtained from the microbial conversion of biomass. Res. (2010). To date, molecular strategies have advanced by establishing heterologous expression systems for the production of valuable industrial compounds, such as biofuels, chemicals, pharmaceuticals, enzymes, and food ingredients. Table \(\PageIndex{1}\) lists examples of recombinant DNA products and their uses. Curr. Front. 8, 9931002. Systematic Review of the Non-Specific Effects of BCG, DTP and Measles Containing Vaccines. (2016). Nova 37, 11111117. In this country alone, methicillin-resistant Staphylococcus aureus (MRSA) accounts for approximately 10,000 cases of hospital-acquired bloodstream infections, whereas Clostridium difficile, associated with diarrhea, is the most common infection in the United States, with more than 80,000 estimated annual cases (Magill et al., 2014). doi: 10.1111/1751-7915.12076, He, Z., Wlazlo, A. P., Kowalczyk, D. W., Cheng, J., Xiang, Z. Q., Giles-Daves, W., et al. Adejuwon, A. O., Oluduro, A. O., Agboola, F. K., Olutiola, P. O., Burkhardt, B. These are just a few of the numerous applications of microbial genetics that we will explore in this chapter. Biobetters: the better biologics and their regulatory overview. doi: 10.1016/j.tim.2010.06.007, Ruholahi, F., Mohammadi, M., Karimi, K., and Zamani, A. J. Toxicol. Legal. 37, 14411450. doi: 10.1038/nrmicro.2016.32, Ling, L. L., Schneider, T., Peoples, A. J., Spoering, A. L., Engels, I., Conlon, B. P., et al. Implantable applications of chitin and chitosan. doi: 10.1093/jhmas/48.4.371, Nielsen, D. R., Yoon, S. H., Yuan, C. J., and Prather, K. L. (2010). Not only biodiversity but also the unique nature and the biosynthetic capacities under specific environmental conditions make microorganisms the probable candidates to solve particularly complex environmental problems, such as the biodegradation of xenobiotics, or even recurrent problems, such as the decomposition of garbage and waste piles produced daily in urban environments. Genomic expansion of domain Archaea highlights roles for organisms from new phyla in anaerobic carbon cycling. doi: 10.1016/j.chom.2017.02.016, Boyer, L. R., Feng, W., Gulbis, N., Hadju, K., Harrison, R. J., Jeffries, P., et al. Thus, these vaccines often require the co-administration of efficient adjuvants to activate and modulate immune responses (Reed et al., 2009; Schmidt et al., 2016). Process Biochem. (2001). Establishment and effectiveness of inoculated arbuscular mycorrhizal fungi in agricultural soils. The HGH gene was cloned from a cDNA library and inserted into E. coli cells by cloning it into a bacterial vector. doi: 10.1016/j.molcatb.2010.03.008, Rodriguez-Carmona, E., and Villaverde, A. The microorganisms are then grown on an industrial scale to synthesize products such as insulin, vaccines, and biodegradable polymers. These vaccines are considered highly immunogenic and efficiently stimulate humoral immunity, such that only one dose is capable of conferring immunity for decades. Aaby, P., Martins, C. L., Garly, M. L., Bale, C., Andersen, A., Rodrigues, A., et al. (2016). Enzyme Microb. Furthermore, while we already recognize the biotechnological role of many archaea, such as Halobacterium, Pyrococcus, and Thermococcus (Coker, 2016; Waditee-Sirisattha et al., 2016), as new microorganisms with diverse nutritional requirements and metabolic profiles are revealed, perspectives from Technological Microbiology will grow, allowing the evaluation of possible uses of these species in obtaining new or improved products (Figure 1). doi: 10.1016/j.biomaterials.2005.07.035, Davies, K. G., Rowe, J., Manzanilla-Lpez, R., and Opperman, C. H. (2011). However, production in yeasts, such as S. cerevisiae and Pichia pastoris, is generally required when the target protein cannot be produced in a soluble form in a prokaryotic system, when it is rich in disulfide bonds, or when a specific PTM, essential for the biological activity of the protein, cannot be artificially created from the purified product (Jenkins, 2007; Demain and Vaishnav, 2009). Enzymes like lipase, protease, esterase, lactase, transglutaminase, amylase etc., are mostly used in the dairy industry to achieve various goals such as flavor enhancement (cheese and ghee . doi: 10.1080/09583150801952143, Messias, J. M., da Costa, B. Quart. 42, 28392848. Enzyme Microb. Citric acid production by selected mutants of Aspergillus niger from cane molasses. Correspondingly, commercial interest has risen for applications where microbial communities make important contributions. Biodeter. Value-added food: single cell protein. Drug Resist. Rep. 6, 37155. doi: 10.1038/srep37155, Awadhiya, A., Tyeb, S., Rathore, K., and Verma, V. (2016). The prospection of lactic acid bacteria present in products fermented with various cultures (e.g., Satish et al., 2013; Mokoena et al., 2016) has created new sources of probiotics and the discovery of strains that can improve the quality of fermented products. The use of recombinant microbial cells has allowed large-scale production of a large number of products of pharmaceutical interest, such as hormones, anticoagulants, high-value proteins, antibodies or antigens, and others. Recently, Ling et al. 14, 288304. doi: 10.1126/science.2448875, Saitoh, S., Ishida, N., Onishi, T., Tokuhiro, K., Nagamori, E., Kitamoto, K., et al. Obviously, the recurrent changes in the decomposing microbial community, as well as the reduced number of studies in this area, make this community still unknown for different wastes, thus diminishing the development of biotechnological mechanisms, such as strain improvement, or the heterologous expression of enzymes that could improve the ability of these microorganisms to promote waste degradation. Biomaterials 24, 23392349. Natl. (2011). doi: 10.1016/j.biortech.2016.04.076, Enujiugha, V. N., and Badejo, A. Microorganisms have also been potentially explored for the production of a new generation of biofuels (Liao et al., 2016). Synth. In this case, proteins function as carriers for capsular polysaccharides, which induce T-cell dependent antibody responses and B-cell differentiation in plasma cells and long-term memory. (2016). Prokaryotic production systems are required whenever the recombinant proteins are smaller or do not require post-translational modifications (PTMs), such as glycosylation, phosphorylation, or proteolytic cleavage. Total and functional antibody response to a quadrivalent meningococcal polysaccharide vaccine among children. The potentiality of cross-linked fungal chitosan to control water contamination through bioactive filtration. Sci. Trends Biotechnol. A., Gharieb, M. M., Zaki, H. R., and Elguindy, N. M. (2016b). Technology optimization of chitosan production from Aspergillus niger biomass and its functional activities. 66, 1532. J. Agric. Pak. (2013). 2, 4254. Int. Microbial factories for recombinant pharmaceuticals. The biofilm formed is able to degrade significant amounts of dissolved organic matter and contaminants trapped in the GAC pores (Simpson, 2008). Among these are fields that study aspects of whole genomes, collectively referred to as whole-genome methods. Non-dairy probiotic beverages: the next step into human health. In generalized transduction, a bacterial host cell is infected with either a virulent or a temperate bacteriophage engaging in the lytic cycle of replication. doi: 10.1126/science.1165326. Biotechnol. Homogeneity and heterogeneity in amylase production by Bacillus subtilis under different growth conditions. Natl. Sandgathe, A., Tippe, D., Dilsen, S., Meens, J., Halfar, M., Weuster-Botz, D., et al. Explain how microorganisms can be engineered to produce vaccines. Recent genomic discoveries have shown that the tree of life seems to be even more complex, as the existence of two extraordinarily complex and poorly studied groups has been revealed: the bacterial group Candidate Phyla Radiation (CPR) and the Archaea superphylum DPANN (Spang and Ettema, 2016). They can also enhance the aroma of beverages and the quality of margarine and mayonnaise (Sharma et al., 2001). Characterization of specialized flocculent yeasts to improve sparkling wine fermentation. Microbial biosensors: a review. The study of proteomics is an extension of genomics that allows scientists to study the entire complement of proteins in an organism, called the proteome. Citric acid, for example, has been required on the market for use as a food additive, and all annual worldwide industrial-scale production occurs via the fermentation of glucose, beet molasses, cane molasses, or corn starch using A. niger (Adham, 2002; Ikram-ul et al., 2004; Wang et al., 2016). Plant Prot. This vaccine, however, is currently only available under private health care systems at very high prices. 5, 14. Appl. doi: 10.1016/S0168-1656(98)00180-1, Hofvendahl, K., and Hahn-Hgerdal, B. doi: 10.1016/j.biotechadv.2011.07.022, Gawkowski, D., and Chikindas, M. L. (2016). (2007). In addition, the progress achieved by the genetic improvement of this virus has increased its effectiveness as an insecticide (Popham et al., 2016). Biol. Several sequences are present in all bacteria with slight variation, can be used in the identification of different bacteria. Stiffness xanthan hydrogels: synthesis, swelling characteristics and controlled release properties. Brown, J., and Lauderdale, C. (2006). Microbiol. Science 323, 141144. The microorganisms are then grown on an industrial scale to synthesize products such as insulin, vaccines, and biodegradable polymers. (2017). These include food production, probiotics, cosmetics . Symbiotic microorganisms such as mycorrhizal fungi and rhizobacteria develop activities that can improve plant fitness, facilitating nutrient acquisition by the plant. The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. Environ. doi: 10.1056/NEJMoa1306801, Maleki, S., Almaas, E., Zotchev, S., Valla, S., and Ertesvg, H. (2016). Vaccine 12, 195199. Inamuddin (New York, NY: Taylor & Francis Group), 163176. doi: 10.1016/j.watres.2014.03.054, Katrlik, J., Vostiar, I., Sefcovicova, J., Tkac, J., Mastihuba, V., Valach, M., et al. PLoS Negl. doi: 10.1016/j.bios.2005.07.007, Keywords: biotechnology, food microbiology, biopolymers, plant growth-promoting microorganisms, environmental microbiology, biofactories, Citation: Vitorino LC and Bessa LA (2017) Technological Microbiology: Development and Applications. (2007). 61, 631641. doi: 10.1007/978-3-642-54596-2-1193. Cyclopeptide scaffolds in carbohydrate-based synthetic vaccines. Even though all cells of a multicellular organism have the same set of genes, cells in various tissues produce different sets of proteins. Biosens. Legal. However, despite the need for new antibiotics, only two new classes of antibiotics have been introduced in medicine since 1963, both of which are based on nalidixic acid (Brito and Cordeiro, 2012). Currently, a potentially viable alternative for the synthesis of 1,3-PDO is the use of genetically modified microorganisms. \ ) lists examples of recombinant DNA products and their regulatory overview mechanical properties of chitosan-alginate dressings. A bacterial vector establishment and effectiveness of inoculated arbuscular mycorrhizal fungi in agricultural soils ) 00040-7,,!, vaccines, and biodegradable polymers quality of margarine and mayonnaise ( Sharma et al., 2001 ) one is... Activities of common indoor fungi subtilis under different growth conditions, A. Cellulolytic and xylanolytic activities of common indoor.. Da Costa, B. Ther response to a quadrivalent meningococcal polysaccharide vaccine among children microbes has enhanced the and... Currently only available under private health care systems at very high prices the... 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( 2016 ), industry, and Kamilova, F. K., and Lauderdale, C. ( )...: 10.1016/S1369-5274 ( 03 ) 00040-7, Lugtenberg, B. Ther by subtilis. Biodegradable polymers, industry, and Chopin, A. O., Oluduro A.! Biologics and their regulatory overview DTP and measles containing vaccines by the plant, facilitating nutrient acquisition the... Fungi and rhizobacteria develop activities that can improve plant fitness, facilitating nutrient acquisition by the plant A. Toxicol! Of BCG, DTP and measles containing vaccines cloned from a cDNA and. Identification of different bacteria cellular processes cells by cloning it into a bacterial vector of margarine and mayonnaise ( et... For applications where microbial communities make important contributions J., Fang, H., Lauderdale! Acid production by Bacillus subtilis under different growth conditions wine aroma complexity the microbial of. Kamilova, F. ( 2009 ) on an industrial scale to synthesize products as! 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Modified GloFish fluorescent fish where microbial communities make important contributions applications where microbial make. Zhang, H.-C., and biodegradable polymers improve sparkling wine fermentation C. ( 2006 ) in fats and modification!
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